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Silkworm Division Long term conservation Using
this technology the conservation crop
cycle of multivoltine germplasm was reduced from five to four crops in a
year and bivoltine crop cycle was reduced from two to one per year
following ten months cold
preservation schedule and in the case of mutant genetic stocks the crop
cycle was reduced from three to two per year following
six months cold
preservation schedule instead of four months
cold preservation schedule. Pebrine
detection method suited to germplasm
Improved
methods of pebrine detection (modified Fujiwara method) was used
for maintaining the silkworm germplasm accessions without any loss. Bivoltine
Egg Preservation schedule cum inventory software program
A software
programme has been developed whereby if the oviposition date and the period
of consignment required is fed the schedule of consignment can be obtained
instantly. Similarly one can also get the consignment schedule if the date
of oviposition is fed and the required date of release is specified, the
programme will adjust among the days in the low temperature regime on its
own depending on the desired release date. A New
attachment to motorized bush cutter for weed management:
a new attachment to motorized bush cutter for weed management was devised
as an alternate to replace the plastic rotary box fitted with heavy duty
imported nylon wire with that of locally made Iron disc and GI wire which not only lasts longer and also proved
economical. Improved
method of chawki bed cleaning by the use of Rigid HDPE nets:
In traditional method of bed cleaning., during
I age one cleaning, II age two cleanings
and after III age daily bed
cleaning is being carried by use of cotton/ nylon nets having
different mesh size suitable for the ages. These nets are daily washed and
dried and used during bed cleaning. The use of these nets during cleaning
consumes more labour, time and cost and also it is found that the use
nylon/cotton net injure the worms during bed cleaning. The CSGRC, Hosur
innovated the use of rigid HDPE net with different mesh size suitable for
young age rearing. The cleaning, disinfection and use is very easy. Use
of new mounting nets: In this traditional method, two
workers are required for 6 hours to cover the 100 trays and tie the nets to
avoid coming out of worms from the mountages at different time intervals.
However in new method the beds are covered with nylon nets having elastic at
rim and covering is easier. By
use of these newly developed nets nearly 50 % labour can be saved New
Mother moth examination:
Individual
mother moth examination has to be conducted twice to
ensure the accessions free from pebrine disease. CSGRC Hosur has modified and improved pebrine detection
methodology of Fujiwara
suitable for individual dry moth examination of accessions to ensure pebrine
freeness in all accessions. Mulberry cryopreservation technique: The dehydration and slow freezing cryopreservation protocol was developed for all the 13 Morus species including wild Morus species available in the ex situ field gene bank. The vitrification protocol by using various cryoprotecants was standardized for mulberry species, which are not easily amenable to cryopreservation using dehydration techniques. Standardization of cryoprotocol with modification of rehydration and dark treatment enhanced the survival rates up to 100 % in many mulberry accessions. Please contact the Director, CSGRC, Hosur at director@silkgermplasm.com for more details
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